Difference between revisions of "Part:BBa K395602"

Revision as of 14:01, 27 October 2010

Apple fragrance generator

The apple fragrance generator (BBa_K395602) catalyzes the conversion of 2-methylbutanol and butanol to 2-methybutyl acetate and butyl acetate that has a apple fragrance. The biosynthetic device is composed of two devices:fragrance enzyme generator [http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 (BBa_K395601)] and a T7 terminator (BBa_K395600).

Apple fragrance expression device

Gas chromatography analysis of apple fragrance expression device

(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.

(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1765
Illegal NotI site found at 1666
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 195
Illegal BamHI site found at 1635
Illegal XhoI site found at 1675
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 205
Illegal AgeI site found at 1419
1000
COMPATIBLE WITH RFC[1000]

Revision as of 12:20, 27 October 2010 (view source) Matsubara (Talk \| contribs) ← Older edit		Revision as of 14:01, 27 October 2010 (view source) Matsubara (Talk \| contribs) Newer edit →
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−	The apple fragrance generator (BBa_K395602) catalyzes the conversion of 2-methylbutanol and butanol to 2-methybutyl acetate and butyl acetate that has a apple fragrance. The biosynthetic device is composed of two devices:fragrance enzyme generator (BBa_K395601) and a T7 terminator (BBa_K395600).	+	The apple fragrance generator (BBa_K395602) catalyzes the conversion of 2-methylbutanol and butanol to 2-methybutyl acetate and butyl acetate that has a apple fragrance. The biosynthetic device is composed of two devices:fragrance enzyme generator [http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 (BBa_K395601)] and a T7 terminator (BBa_K395600).