Difference between revisions of "Part:BBa K320014:Design"
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===Source=== | ===Source=== | ||
| − | This part has been designed from parts | + | This part has been designed from the following parts: <partinfo>BBa_K320003</partinfo>, <partinfo>BBa_K320007</partinfo> and <partinfo>BBa_K320009</partinfo>. |
===References=== | ===References=== | ||
Latest revision as of 13:11, 27 October 2010
S + P25 + P28 + Ori + Tet
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
Illegal NheI site found at 827 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2699
Illegal AgeI site found at 2859 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1537
Design Notes
The P protein sequences were very AT-rich and not optimized for Asaia and E.Coli. No expression took place.
Source
This part has been designed from the following parts: BBa_K320003, BBa_K320007 and BBa_K320009.