Difference between revisions of "Part:BBa K395402"
Line 2: Line 2:
 
<partinfo>BBa_K395402 short</partinfo>
 
<partinfo>BBa_K395402 short</partinfo>
  
−
In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part. <br>
+
In order to measure LacI(BBa_K395400), we designed this part. <br>
−
We confirmed that lacIM1 shows weaker repression than LacIWT.  Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])<br>
+
We confirmed that lacIM1 shows weaker repression than LacIWT.  Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.
 +
(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])<br>
 
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.
 
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.
  
Line 11: Line 12:
 
[[Image:Tokyotech_LacIM1_system_ver4.png|left|thumb|300px|Figure3-2-1. ]]
 
[[Image:Tokyotech_LacIM1_system_ver4.png|left|thumb|300px|Figure3-2-1. ]]
 
[[Image:Tokyotech_LacIM1_data_ver2.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG.  This work is done by Mitsuhiko Odera ]]
 
[[Image:Tokyotech_LacIM1_data_ver2.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG.  This work is done by Mitsuhiko Odera ]]
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:08, 27 October 2010

pBad/araC-lacI

In order to measure LacI(BBa_K395400), we designed this part.
We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1. 

(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])

We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.



Figure3-2-1.
Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961