Difference between revisions of "Part:BBa K395402"
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<partinfo>BBa_K395402 short</partinfo>
 
<partinfo>BBa_K395402 short</partinfo>
  
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In order to measure the BBa_K082026/BBa_K395400, we designed this part.
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In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part. <br>
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We confirmed that lacIM1 shows weaker repression than LacIWT.  Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])<br>
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We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.
  
  
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[[Image:Tokyotech_LacIM1_system.png|left|thumb|300px|Figure3-2-1. ]]
 
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[[Image:Tokyotech_LacIM1_data.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG.  This work is done by Mitsuhiko Odera ]]
 
  
  
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[[Image:Tokyotech_LacIM1_system_ver4.png|left|thumb|300px|Figure3-2-1. ]]
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[[Image:Tokyotech_LacIM1_data_ver2.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG.  This work is done by Mitsuhiko Odera ]]
  
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We confirmed that lacIM1 shows weaker repression than LacIWT.
 
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(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:07, 27 October 2010

pBad/araC-lacI

In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part.
We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.



Figure3-2-1.
Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961