Difference between revisions of "Part:BBa K395402:Experience"
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===Applications of BBa_K395402=== | ===Applications of BBa_K395402=== | ||
| − | In order to measure | + | In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part. <br> |
| + | We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1. | ||
| + | (→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])<br> | ||
| + | We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2. | ||
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| + | [[Image:Tokyotech_LacIM1_system_ver4.png|left|thumb|300px|Figure3-2-1. ]] | ||
| + | [[Image:Tokyotech_LacIM1_data_ver2.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera ]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:05, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K395402
In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part.
We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.
(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.
User Reviews
UNIQ2b8379f5f92bb766-partinfo-00000000-QINU UNIQ2b8379f5f92bb766-partinfo-00000001-QINU