Difference between revisions of "Part:BBa K395400"

Revision as of 12:48, 27 October 2010

RBS-lacIM1-ter

Though LacIM1(BBa_K082026) has been registered, sequence data of BBa_K082026 is incorrect. Therefore, we resistered this part again.
LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter .

We confirmed that lacIM1 shows weaker repression than LacIWT.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We ligated BBa_K395400 together with Pbad/araC promoter. Then, we used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.

Figure3-2-1.

Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Therefore, we resistered this part again. <br>
 LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter . <br><br>
-We confirmed that lacIM1 shows weaker repression than LacIWT.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
+We confirmed that lacIM1 shows weaker repression than LacIWT.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])<br>
+We ligated BBa_K395400 together with Pbad/araC promoter.
+Then, we used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig.  3-2-1).
+Fluorescence was measured, and the result is shown in Fig. 3-2-2.