Difference between revisions of "Part:BBa K320008:Design"

(→‎Source)
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===Design Notes===
 
===Design Notes===
−
Ligation of both part quote below, which were ligation of BBa_K320001/BBa_K320002 and BBa_K320000/BBa_P1005.
+
Ligation of parts mentioned below (which were ligations themselves).
  
 
===Source===
 
===Source===

Revision as of 12:43, 27 October 2010

Constitutive expression of Immunotoxin in Asaia and resistance to Kanamycine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2297
    Illegal AgeI site found at 2457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1135


Design Notes

Ligation of parts mentioned below (which were ligations themselves).

Source

Both BioBrick we used were BBa_K320006 and BBa_K320004.

References