Difference between revisions of "Part:BBa K317023"

 
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<partinfo>BBa_K317023 short</partinfo>
 
<partinfo>BBa_K317023 short</partinfo>
  
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Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome.
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The wildtype of Antigen 43 contains 6 PstI sites but this part does not contain any of them. So, this parts can be used as a normal BioBrick which is able to ligate 2 parts by 3A assembly.
  
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This parts is a generator composed of RBS (BBa_B0034), Antigen 43 (BBa_K317008) and Double terminator (BBa_B0015).
  
  

Revision as of 12:12, 27 October 2010

RBS-Antigen 43-Double terminator

Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome. The wildtype of Antigen 43 contains 6 PstI sites but this part does not contain any of them. So, this parts can be used as a normal BioBrick which is able to ligate 2 parts by 3A assembly.

This parts is a generator composed of RBS (BBa_B0034), Antigen 43 (BBa_K317008) and Double terminator (BBa_B0015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 727
    Illegal NgoMIV site found at 1951
    Illegal NgoMIV site found at 2461
    Illegal NgoMIV site found at 2482
    Illegal AgeI site found at 1477
    Illegal AgeI site found at 2221
    Illegal AgeI site found at 2635
    Illegal AgeI site found at 2877
  • 1000
    COMPATIBLE WITH RFC[1000]