Difference between revisions of "Part:BBa K395400"

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Though LacIM1(BBa_K082026) has been registered, sequence data of BBa_K082026 is incorrect.
 
Though LacIM1(BBa_K082026) has been registered, sequence data of BBa_K082026 is incorrect.
 
Therefore, we resistered this part again. <br>
 
Therefore, we resistered this part again. <br>
LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter .   
+
LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter . <br>
 +
  We confirmed that lacIM1 shows weaker repression than LacIWT.
 +
 
 +
(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
 +
 
  
 
[[Image:Tokyotech_LacIM1_system.png|left|thumb|300px|Figure3-2-1. ]]
 
[[Image:Tokyotech_LacIM1_system.png|left|thumb|300px|Figure3-2-1. ]]
 
[[Image:Tokyotech_LacIM1_data.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG.  This work is done by Mitsuhiko Odera ]]
 
[[Image:Tokyotech_LacIM1_data.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG.  This work is done by Mitsuhiko Odera ]]
  
We confirmed that lacIM1 shows weaker repression than LacIWT.
 
 
(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
 
  
  

Revision as of 11:58, 27 October 2010

RBS-lacIM1-ter

Though LacIM1(BBa_K082026) has been registered, sequence data of BBa_K082026 is incorrect. Therefore, we resistered this part again.
LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter .

We confirmed that lacIM1 shows weaker repression than LacIWT.

(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])


Figure3-2-1.
Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]