Difference between revisions of "Part:BBa K395602"
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[[Image:Tokyotech apple fragrance GC.png|600px|center|thumb|Gas chromatography analysis of apple fragrance expression device]]
 
[[Image:Tokyotech apple fragrance GC.png|600px|center|thumb|Gas chromatography analysis of apple fragrance expression device]]
 
(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.
 
(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.
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(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 more information])
  
  

Revision as of 11:52, 27 October 2010

Apple fragrance generator

The apple fragrance generator (BBa_K395602) catalyzes the conversion of 2-methylbutanol and butanol to the odor 2-methybutyl acetate and butyl acetate that has a apple fragrance. The biosynthetic device is composed of two devices:fragrance enzyme generator (BBa_K395601) and a T7 terminator (BBa_K395600).


Apple fragrance expression device


Gas chromatography analysis of apple fragrance expression device

(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.

(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 more information])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1765
    Illegal NotI site found at 1666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
    Illegal BamHI site found at 1635
    Illegal XhoI site found at 1675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 205
    Illegal AgeI site found at 1419
  • 1000
    COMPATIBLE WITH RFC[1000]