Difference between revisions of "Part:BBa I13453"
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See this OpenWetWare article on [http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare pBAD and lac promoters] for additional usage and biology information
 
See this OpenWetWare article on [http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare pBAD and lac promoters] for additional usage and biology information
  
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===Characterization===
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For the 2009 iGEM competition, British_Columbia characterized <partinfo>I13453</partinfo> in the context of a [https://parts.igem.org/PBAD_Promoter_Family pBAD promoter family]. For the results of this characterization, see [[Part:BBa_I13453:British_Columbia|here]].
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_I13453 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_I13453 SequenceAndFeatures</partinfo>
  
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===Characterization===
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For the 2009 iGEM competition, British_Columbia characterized <partinfo>I13453</partinfo> in the context of a [https://parts.igem.org/PBAD_Promoter_Family pBAD promoter family]. For the results of this characterization, see [[Part:BBa_I13453:British_Columbia|here]].
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For the 2010 iGEM competition, Tec-Monterrey characterized <partinfo>I13453</partinfo> again, using a construct of GFP reporter after the pBad promoter. The transfer function was modeled with a Hill equation.
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<!-- -->
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[[Image:pbadwt.png|center|frame|Figure 1. Transfer function of <partinfo>BBa_I13453</partinfo>. Points represent individual measurements. The line is of a Hill equation fitted to our data.]]
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<center>
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(d[GFP]/dt)/OD<sub>600</sub> = C+A*X<sup>n</sup>/(X<sup>n</sup>+K<sup>n</sup>)
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{|{{Table}}
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!Experiment
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!Characteristic
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!Value
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|-
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|rowspan="4"|[[#Transfer Function|'''Transfer Function''']]
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|''Basal rate (C)''
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|1 d[GFP]/dt
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|-
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|''Gain (A)''
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|36 d[GFP]/dt
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|-
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|''Hill coefficient (n)''
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|2.16
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|-
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|''Switch Point (K)''
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|2.8 [ara] (µM)
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|-
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|}
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</center>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 11:45, 27 October 2010

Pbad promoter

PBad promoter from I0500 without AraC.


Usage and Biology

Has been used as a second promoter in a system containing BBa_I0500 (PBad+AraC). In this system, it showed behavior qualitatively indistinguishable from the BBa_I0500 copy of PBad. Has not been tested independent of AraC. A second part, BBa_I13458, should allow decoupling of PBad and AraC.

See this OpenWetWare article on [http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare pBAD and lac promoters] for additional usage and biology information


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

For the 2009 iGEM competition, British_Columbia characterized BBa_I13453 in the context of a pBAD promoter family. For the results of this characterization, see here.

For the 2010 iGEM competition, Tec-Monterrey characterized BBa_I13453 again, using a construct of GFP reporter after the pBad promoter. The transfer function was modeled with a Hill equation.

Figure 1. Transfer function of BBa_I13453. Points represent individual measurements. The line is of a Hill equation fitted to our data.

(d[GFP]/dt)/OD600 = C+A*Xn/(Xn+Kn)

Experiment Characteristic Value
Transfer Function Basal rate (C) 1 d[GFP]/dt
Gain (A) 36 d[GFP]/dt
Hill coefficient (n) 2.16
Switch Point (K) 2.8 [ara] (µM)