Difference between revisions of "Part:BBa K427003"
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Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction. | Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The activation of the promoter requires the holenzyme σ70, which normally binds to the -35 and -10 consensus sequences, the promoter has the -10 but does not have the -35 sequences instead it has another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzyme σ70 recognizes the promoter and transcription begins. | ||
| + | This part has no special safety considerations. | ||
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===<span class='h3bb'>Sequence and Features</span>=== | ===<span class='h3bb'>Sequence and Features</span>=== | ||
Latest revision as of 11:14, 27 October 2010
Pm promoter of Mu bacteriophage
Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction.
Usage and Biology
The activation of the promoter requires the holenzyme σ70, which normally binds to the -35 and -10 consensus sequences, the promoter has the -10 but does not have the -35 sequences instead it has another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzyme σ70 recognizes the promoter and transcription begins.
This part has no special safety considerations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Kumaraswami, M., & Howe, M. a.-W. (2004). Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.