Difference between revisions of "Part:BBa K389017"
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|style="border-style: solid; border-width: 1px"| Not induced by | |style="border-style: solid; border-width: 1px"| Not induced by | ||
|style="border-style: solid; border-width: 1px"| | |style="border-style: solid; border-width: 1px"| | ||
| − | + | Capsaicin | |
| − | + | ||
| − | + | Dopamine | |
| − | + | ||
| + | Homovanillic acid | ||
| + | |||
| + | 3-Methoxytyramine | ||
|} | |} | ||
</center> | </center> | ||
Revision as of 10:57, 27 October 2010
VirA/G signaling system without read-out
This BioBrick is a VirA/G signaling system from Agrobacterium tumefaciens without read-out. It contains an unmutated virA gene and a virG gene that works without rpoA from A. tumefaciens and a vir promoter. In the presence of the inducer acetosyringone VirA phosphorylates VirG and this activates the vir promoter.
Parameters
Characterizations of this part were made with BBa_K389015 and BBa_K389016. Look at the description of these parts to get more detailed information about the VirA/G signaling system. In the following table, some important parameters of the VirA/G signaling system are summarized:
| Parameter | Result |
|---|---|
| Input | Acetosyringone |
| Output | PoPS |
| Switch point | ~ 30 µM acetosyringone |
| Fully induced | > 150 µM acetosyringone |
| Increased expression when induced | > 2.2 fold (compared to uninduced system) |
| Hill coefficient | positive cooperation (> 1) |
| Not induced by |
Capsaicin Dopamine Homovanillic acid 3-Methoxytyramine |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768