Difference between revisions of "Part:BBa K427000"

 
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PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences.  
 
PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences.  
  
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===Usage and Biology===
 
===Usage and Biology===
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It can be used to promote the transcription of DNA sequences coded in the antisense strand of DNA it can also be used to build a jammer device in which the translation of a protein is stopped because of the mRNA produced by the sense and antisense strand are complimentary.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K427000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K427000 SequenceAndFeatures</partinfo>
  
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===References===
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<partinfo>BBa_K206001</partinfo> part created by British Columbia team in 2009.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K427000 parameters</partinfo>
 
<partinfo>BBa_K427000 parameters</partinfo>
 
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Revision as of 10:51, 27 October 2010

PBad Weak Reverse

PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences.


Usage and Biology

It can be used to promote the transcription of DNA sequences coded in the antisense strand of DNA it can also be used to build a jammer device in which the translation of a protein is stopped because of the mRNA produced by the sense and antisense strand are complimentary.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 61
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

BBa_K206001 part created by British Columbia team in 2009.