Difference between revisions of "Part:BBa K313035"

 
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<partinfo>BBa_K313035 short</partinfo>
 
<partinfo>BBa_K313035 short</partinfo>
  
 
This is a device that aims to test the recombination ability of Hin DNA invertase.
 
This is a device that aims to test the recombination ability of Hin DNA invertase.
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This part is composed of "T7 promoter-lox71-Tet resistance(reverse)-rbs(reverse)-lox66-double terminator."
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lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed. So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.
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Revision as of 10:48, 27 October 2010

Hin invertase assay device

This is a device that aims to test the recombination ability of Hin DNA invertase. This part is composed of "T7 promoter-lox71-Tet resistance(reverse)-rbs(reverse)-lox66-double terminator."

lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed. So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]