Difference between revisions of "Part:BBa K320005:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K320005=== | ===Applications of BBa_K320005=== | ||
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| + | We used this BioBrick to characterize the promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By monitoring the CFP expression in time we could measure the activity of the promoter. Moreover to see if the presence of the CFP hindrate the growth of the clones we measured the growth curve.<br /> | ||
| + | Both measurements were done contemporaneously in E<i>.coli</i> DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br /> | ||
| + | The control for the autofluorescence and the growthcurve were done with a wildtype strain of DH5a. | ||
| + | Unfortunately we can't compare our measurements with a well known promoter.<br /> | ||
| + | Further characterisation will focus on the measurement of the promoter's activity in Asaia. | ||
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| + | [[Image:EPFL OD prom.png|800px|center]] | ||
| + | [[Image:EPFL_CFP_prom.png|800px|center]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 09:53, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K320005
We used this BioBrick to characterize the promoter we want to use in Asaia BBa_K320002. By monitoring the CFP expression in time we could measure the activity of the promoter. Moreover to see if the presence of the CFP hindrate the growth of the clones we measured the growth curve.
Both measurements were done contemporaneously in E.coli DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.
The control for the autofluorescence and the growthcurve were done with a wildtype strain of DH5a.
Unfortunately we can't compare our measurements with a well known promoter.
Further characterisation will focus on the measurement of the promoter's activity in Asaia.
User Reviews
UNIQ8635fcad78dde61d-partinfo-00000001-QINU UNIQ8635fcad78dde61d-partinfo-00000002-QINU