Difference between revisions of "Part:BBa K494003"

Revision as of 09:31, 27 October 2010

Exemplary insert for BBa_K494001 (His-Term)

This is a ready to use part consisting of our screening backbone BBa_K494001, a modified version of pSB1A10, the PBad Promotor BBa_I13453, the His Terminator and the reporter protein mCherry BBa_J06702 including RBS and double Terminator BBa_B0015.
This part belongs to the screening system for evaluation of individual switches constructed on the principle of bioLOGICS, in this case BBaK494003 may serve as a reference to compare results with those presented by the TU Munich 2010 iGEM Team. Together with BBaK494004 (Terminator plus small it is a setup to evaluate termination and antitermination efficiency of the His-Terminator.
Furthermore BBaK494003 can also be used as a reference in any other measurement concerning Terminator efficiency. The His-Terminator was found to be a strong terminator with allowed no detectable transcription of the following mCherry gene.
The His-Terminator (which does not exist as an individual BioBrick since there are enough thoroughly tested Terminators yet in the registry) is based on an attenuator stem loop in front of the His-operon of Salmonella enterica.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K494003 short</partinfo>
-Exemplary insert for use with BBa_K494001.
+This is a ready to use part consisting of our screening backbone BBa_K494001, a modified version of pSB1A10, the PBad Promotor BBa_I13453, the His Terminator and the reporter protein mCherry BBa_J06702 including RBS and double Terminator BBa_B0015. <br>
-Here we present a ready to use composite part consisting of our screening system pSB1A10mod BBa_K494001, the PBad Promotor BBa_I13453, the His Terminator and the reporter protein mCherry BBa_J06702 including RBS and double Terminator BBa_B0015. This part belongs to the screening system and is intended as negative control.
+This part belongs to the screening system for evaluation of individual switches constructed on the principle of bioLOGICS, in this case BBaK494003 may serve as a reference to compare results with those presented by the TU Munich 2010 iGEM Team. Together with BBaK494004 (Terminator plus small  it is a setup to evaluate termination and antitermination efficiency of the His-Terminator. <br>
+Furthermore BBaK494003 can also be used as a reference in any other measurement concerning Terminator efficiency. The His-Terminator was found to be a strong terminator with allowed no detectable transcription of the following mCherry gene.
-The His terminator was supposed to work as a toggle switch; for more details check link
+<br>
+The His-Terminator (which does not exist as an individual BioBrick since there are enough thoroughly tested Terminators yet in the registry) is based on an attenuator stem loop in front of the ''His-operon'' of ''Salmonella enterica''.
 <!-- Add more about the biology of this part here