Difference between revisions of "Part:BBa K337041"

 
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<partinfo>BBa_K337041 short</partinfo>
 
<partinfo>BBa_K337041 short</partinfo>
  
CMV promoter driving a Tet Repressor followed by BGH terminator. The construct is flanked by rc BGH terminators to introduce BamHI sites. XhoI/XmaI sites were introduced into the 3'UTR of the TetR for enabling easy introduction of synthetic microRNA binding sites.
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CMV promoter driving a Tet Repressor followed by BGH terminator. The construct is flanked by rc BGH terminators to introduce BamHI sites. XhoI/XmaI sites were introduced into the 3'UTR of the TetR for enabling easy introduction of synthetic microRNA binding sites (BB-2, RFC12).
  
 
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Latest revision as of 09:21, 27 October 2010

BGH(rc)_CMV/TetR/BGH(fw)/BGH(rc)

CMV promoter driving a Tet Repressor followed by BGH terminator. The construct is flanked by rc BGH terminators to introduce BamHI sites. XhoI/XmaI sites were introduced into the 3'UTR of the TetR for enabling easy introduction of synthetic microRNA binding sites (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]