Difference between revisions of "Part:BBa K395301"
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This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of P''OmpC(WT)''BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. ''OmpC(C)'' promoter consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
 
This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of P''OmpC(WT)''BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. ''OmpC(C)'' promoter consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
  
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[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. If you add E0240 as a reporter, this result is gained. Induction of new'' OmpC'' series in high osmolarity medium This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010]]  
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[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium at 4 hours. We characterized this promoter by attaching it to GFP(E0240)and mearsuring it transcriptional activity through the GFP expression. <br> This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010]]  
  
 
  <!-- Add more about the biology of this part here.
 
  <!-- Add more about the biology of this part here.

Revision as of 09:08, 27 October 2010

PompC(C) Promoter regulated by phosphorylated OmpR

This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of POmpC(WT)BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. OmpC(C) promoter consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. Induction of new OmpC series in high osmolarity medium at 4 hours. We characterized this promoter by attaching it to GFP(E0240)and mearsuring it transcriptional activity through the GFP expression.
This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 24
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]