Difference between revisions of "Part:BBa C0072:Design"

 
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<partinfo>BBa_C0072 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_C0072 SequenceAndFeatures</partinfo>
  
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===Design Notes===
 
  
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===Design Notes===
 
wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.<br/><br/>
 
wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.<br/><br/>
  
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===Source===
 
  
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--What is the source of this part?--
 
  
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===References===
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===Source===
  
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enterobacteriophage p22
  
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===Files===
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===References===

Revision as of 01:22, 5 June 2006


mnt repressor (strong) from Salmonella phage P22 (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.

sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).

Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor
Kendall, KL; Sauer, RT
The Journal of Biological Chemistry, 264(23):13706-13710, 1989



Source

enterobacteriophage p22

References