Difference between revisions of "Part:BBa K313022"

 
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<partinfo>BBa_K313022 short</partinfo>
 
<partinfo>BBa_K313022 short</partinfo>
  
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This is a device that aims to measure the leakiness of terminator BBa_B1007.
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This is a device that aims to measure the leakiness of a terminator BBa_B1007.
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If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
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With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
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The difference between this part and BBa K313021 is the number of the terminator.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:04, 27 October 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1007.

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.

The difference between this part and BBa K313021 is the number of the terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]