Difference between revisions of "Part:BBa K313017"
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This is a device that aims to measure the leakiness of a terminator BBa_B1006.
 
This is a device that aims to measure the leakiness of a terminator BBa_B1006.
 +
 
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
 
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
−
With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator.
+
 
 +
With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:58, 27 October 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1006.

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]