Difference between revisions of "Part:BBa K313019"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K313019 short</partinfo> | <partinfo>BBa_K313019 short</partinfo> | ||
This is a device that aims to measure the leakiness of terminator BBa_B0014 | This is a device that aims to measure the leakiness of terminator BBa_B0014 | ||
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| + | If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. | ||
| + | With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 07:43, 27 October 2010
terminator leakiness assay device
This is a device that aims to measure the leakiness of terminator BBa_B0014
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 534
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 220
- 1000COMPATIBLE WITH RFC[1000]