Difference between revisions of "Part:BBa I742158:Experience"

No review score entered. Tokyo Tech iGEM2010

Our team synthesized zeaxanthin by several kinds of construct to confirm the activity of BioBrick parts designed by Edinburgh 2007. By assembling the crtZ, crtEBIY and appropriate promoters, we constructed new BioBrick parts to synthesize zeaxanthin and compared their function . (→BBa_K395701, BBa_K395702, BBa_K395704)

We constructed two types of zeaxanthin synthetic construct. One is that crtZ(BBa_I742158) and crtEBIY(BBa_K274210) were assembled on a single plasmid.(We call this construct “single plasmid construct”.)The other is that crtZ and crtEBIYwere assembled on different plasmids.(we call this type of constructs “double plasmids construct”.) We made 5 combinations shown in table<1>.

Constructs containing pBad/araC promoter on high-copy vector show the best pruduction and double plasmids construct shows as much pruduction as single plasmid construct under same promoter conbination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that crtEBIY on high-copy vector and crtZ on low-copy vector shows as much expression as construct that crtEBIY on low-copy vector and crtZ on high-copy vector.（Actually, ③ showed a little more production than ⑤.)

figure1