Difference between revisions of "Part:BBa I742158:Experience"
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We made 5 combinations shown in table<1>.<br><br> | We made 5 combinations shown in table<1>.<br><br> | ||
Constructs containing pBad/araC promoter on high-copy vector show the best pruduction and double plasmids construct shows as much pruduction as single plasmid construct under same promoter conbination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that ''crtEBIY'' on high-copy vector and ''crtZ'' on low-copy vector shows as much expression as construct that ''crtEBIY'' on low-copy vector and ''crtZ'' on high-copy vector.(Actually, ③ showed a little more production than ⑤.)<br><br> | Constructs containing pBad/araC promoter on high-copy vector show the best pruduction and double plasmids construct shows as much pruduction as single plasmid construct under same promoter conbination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that ''crtEBIY'' on high-copy vector and ''crtZ'' on low-copy vector shows as much expression as construct that ''crtEBIY'' on low-copy vector and ''crtZ'' on high-copy vector.(Actually, ③ showed a little more production than ⑤.)<br><br> | ||
− | [[Image:tokyotech_Fig. 1.1.7.1.BBa_K395704.jpg|right| | + | [[Image:tokyotech_Fig. 1.1.7.1.BBa_K395704.jpg|right|300px|thumb|figure1]] |
− | [[Image:tokyotech_Fig. 1.1.8.1. zeaxanthin pellet and plate.jpg|left| | + | [[Image:tokyotech_Fig. 1.1.8.1. zeaxanthin pellet and plate.jpg|left|300px|Fig. 1.1.8.1. zeaxanthin pellet and plate]] |
− | [[Image:tokyotech_table. 1.1.1.2.jpg| | + | [[Image:tokyotech_table. 1.1.1.2.jpg|300px|left|table<1>]] |
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Revision as of 07:31, 27 October 2010
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Applications of BBa_I742158
User Reviews
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No review score entered. Tokyo Tech iGEM2010 |
Our team synthesized zeaxanthin by several kinds of construct to confirm the activity of BioBrick parts designed by Edinburgh 2007. By assembling the crtZ, crtEBIY and appropriate promoters, we constructed new BioBrick parts to synthesize zeaxanthin and compared their function . (→BBa_K395701, BBa_K395702, BBa_K395704) |
UNIQc21c503af3f1daf3-partinfo-00000002-QINU