Difference between revisions of "Part:BBa I742158:Experience"
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===User Reviews===
 
===User Reviews===
 
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<I>Tokyo Tech iGEM2010</I>
 
<I>Tokyo Tech iGEM2010</I>
 
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Enter the review inofrmation here.Our team synthesized zeaxanthin by several kinds of construct to confirm the activity of BioBrick parts designed by Edinburgh 2007. By assembling the ''crtZ'', ''crtEBIY'' and appropriate promoters, we constructed new BioBrick parts to synthesize zeaxanthin and compared their function .  
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Our team synthesized zeaxanthin by several kinds of construct to confirm the activity of BioBrick parts designed by Edinburgh 2007. By assembling the ''crtZ'', ''crtEBIY'' and appropriate promoters, we constructed new BioBrick parts to synthesize zeaxanthin and compared their function . (→[https://parts.igem.org/wiki/index.php?title=Part:BBa_K395701 BBa_K395701], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K395702 BBa_K395702], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K395704 BBa_K395704])<br><br>
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(→[https://parts.igem.org/wiki/index.php?title=Part:BBa_K395701 BBa_K395701], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K395702 BBa_K395702], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K395704 BBa_K395704])<br><br>
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We constructed two types of zeaxanthin synthetic construct. One is that ''crtZ''(BBa_I742158) and ''crtEBIY''(BBa_K274210) were assembled on a single plasmid.(We call this construct “single plasmid construct”.)The other is that ''crtZ'' and ''crtEBIY''were assembled on different plasmids.(we call this type of constructs “double plasmids construct”.)
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We constructed two types of zeaxanthin synthetic construct.  
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One is that ''crtZ''(BBa_I742158) and ''crtEBIY''(BBa_K274210) were assembled on a single plasmid.(We call this construct “single plasmid construct”.)
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The other is that ''crtZ'' and ''crtEBIY''were assembled on different plasmids.(we call this type of constructs “double plasmids construct”.)
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We made 5 combinations shown in table<1>.<br><br>
 
We made 5 combinations shown in table<1>.<br><br>
 
Constructs containing pBad/araC promoter on high-copy vector show the best pruduction and double plasmids construct shows as much pruduction as single plasmid construct under same promoter conbination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that ''crtEBIY'' on high-copy vector and ''crtZ'' on low-copy vector shows as much expression as construct that ''crtEBIY'' on low-copy vector and ''crtZ'' on high-copy vector.（Actually, ③ showed a little more production than ⑤.)<br><br>
 
Constructs containing pBad/araC promoter on high-copy vector show the best pruduction and double plasmids construct shows as much pruduction as single plasmid construct under same promoter conbination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that ''crtEBIY'' on high-copy vector and ''crtZ'' on low-copy vector shows as much expression as construct that ''crtEBIY'' on low-copy vector and ''crtZ'' on high-copy vector.（Actually, ③ showed a little more production than ⑤.)<br><br>
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[[Image:tokyotech_Fig. 1.1.8.1. zeaxanthin pellet and plate.jpg|left|290px|Fig. 1.1.8.1. zeaxanthin pellet and plate]]
 
[[Image:tokyotech_Fig. 1.1.8.1. zeaxanthin pellet and plate.jpg|left|290px|Fig. 1.1.8.1. zeaxanthin pellet and plate]]
 
[Image:tokyotech_table. 1.1.1.2.jpg|500px|center|thumb|table<1>]]
 
[Image:tokyotech_table. 1.1.1.2.jpg|500px|center|thumb|table<1>]]
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Revision as of 07:26, 27 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I742158

User Reviews

UNIQ15f47d697e74a9c2-partinfo-00000000-QINU

No review score entered. Tokyo Tech iGEM2010

Our team synthesized zeaxanthin by several kinds of construct to confirm the activity of BioBrick parts designed by Edinburgh 2007. By assembling the crtZ, crtEBIY and appropriate promoters, we constructed new BioBrick parts to synthesize zeaxanthin and compared their function . (→BBa_K395701, BBa_K395702, BBa_K395704)

We constructed two types of zeaxanthin synthetic construct. One is that crtZ(BBa_I742158) and crtEBIY(BBa_K274210) were assembled on a single plasmid.(We call this construct “single plasmid construct”.)The other is that crtZ and crtEBIYwere assembled on different plasmids.(we call this type of constructs “double plasmids construct”.) We made 5 combinations shown in table<1>.

Constructs containing pBad/araC promoter on high-copy vector show the best pruduction and double plasmids construct shows as much pruduction as single plasmid construct under same promoter conbination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that crtEBIY on high-copy vector and crtZ on low-copy vector shows as much expression as construct that crtEBIY on low-copy vector and crtZ on high-copy vector.（Actually, ③ showed a little more production than ⑤.)

figure1
Fig. 1.1.8.1. zeaxanthin pellet and plate

[Image:tokyotech_table. 1.1.1.2.jpg|500px|center|thumb|table<1>]]

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UNIQ15f47d697e74a9c2-partinfo-00000002-QINU