Difference between revisions of "Part:BBa K395146"
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We measured 3OC6HSL production by addition of supernatant from liquid culture of K395146 into a reporter cell which expresses GFP in presence of LuxR/3OC6HSL. Fig1 shows GFP fluorescence increased in presence of the supernatant of liquid culture with LuxI-expression cell, which proved 3OC6HSL was synthesized by K395146. Fig2 shows the relationship between GFP fluorescence and the concentration of 3OC6HSL. Taking account of this graph, the level of activity of K395146 is equal to about 10 nM 3OC6HS. | We measured 3OC6HSL production by addition of supernatant from liquid culture of K395146 into a reporter cell which expresses GFP in presence of LuxR/3OC6HSL. Fig1 shows GFP fluorescence increased in presence of the supernatant of liquid culture with LuxI-expression cell, which proved 3OC6HSL was synthesized by K395146. Fig2 shows the relationship between GFP fluorescence and the concentration of 3OC6HSL. Taking account of this graph, the level of activity of K395146 is equal to about 10 nM 3OC6HS. | ||
Revision as of 07:04, 27 October 2010
constitutive LuxI generator
This is a constitutive LuxI generator using P(Lac)IQ (High Transcription) with RBS and Terminator.
The level of activity of K395146 is equal to about 10 nM 3OC6HSL.
We measured 3OC6HSL production by addition of supernatant from liquid culture of K395146 into a reporter cell which expresses GFP in presence of LuxR/3OC6HSL. Fig1 shows GFP fluorescence increased in presence of the supernatant of liquid culture with LuxI-expression cell, which proved 3OC6HSL was synthesized by K395146. Fig2 shows the relationship between GFP fluorescence and the concentration of 3OC6HSL. Taking account of this graph, the level of activity of K395146 is equal to about 10 nM 3OC6HS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]