Difference between revisions of "Part:BBa K395705"

Revision as of 06:14, 27 October 2010

crtW (beta-carotene ketolase) with rbs

CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.

Part of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.

Fig. 2-1-11-2 astaxanthin pellet and plate

These parts contain crtW (β-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 632
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 715
Illegal SapI site found at 542
Illegal SapI site found at 554

@@ Line 4: / Line 4: @@
 CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
-Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
+Part of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.
 [[Image:tokyotech_registry-metabolite705.jpg|left|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]