Difference between revisions of "Part:BBa K395706"

Revision as of 05:40, 27 October 2010

rbs+crtZW under pBad promoter for double plasmids

This part contains crtZW, which works as the astaxanthin biosynthesis pathway when add beta-carotene as a substrate.

It is necessary to add either beta-carotene or the synthesis operon.

Fig. 2-1-11-2 astaxanthin pellet and plate

These parts contain crtW (β-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2405
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2488
Illegal SapI site found at 961
Illegal SapI site found at 2315
Illegal SapI site found at 2327

@@ Line 6: / Line 6: @@
 It is necessary to add either beta-carotene or the synthesis operon.
-[[Image:tokyotech_Fig. 2-1-11 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11 astaxanthin pellet and plate]]
+[[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
-[[Image:tokyotech_Fig. 2-1-12 astaxanthin extract.jpg|right|460px|Fig. 2-1-12 astaxanthin extract]]
+[[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right|500px|Fig. 2-1-12-2 astaxanthin extract]]
 [[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]]
-[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|450px|Fig. 2-1-1-3.TLC.jpg]]
+[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|400px|Fig. 2-1-1-3.TLC.jpg]]