Difference between revisions of "Part:BBa K395400"

Revision as of 05:34, 27 October 2010

RBS-lacIM1-ter

LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter [1]. LacIM1 (BBa_K082026) which is the key component in the band-detect network. (Fig. 3-2-1) [1]. Though this part was registered by USTC(2008), its registered sequence is incorrect and it was not well characterized in their wiki. Therefore, we registered this part as BBa_K395400.

Figure3-2-1.

Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera

We confirmed that lacIM1 shows weaker repression than LacIWT.

(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K395400 short</partinfo>
-resister this part since the sequence of BBa_K082026 is wrong.lacIM1 is a codon-modified lacI designed to reduce the likelihood of recombination with the wild-type lacI.lacIM1 represses lac promoter.
-But this repression is lower than that of lacI wild-type.(Subhayu Basu,et al.,2005).
+LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter [1].
+LacIM1 (BBa_K082026) which is the key component in the band-detect network. (Fig. 3-2-1) [1]. Though this part was registered by USTC(2008), its registered sequence is incorrect and it was not well characterized in their wiki.  Therefore, we registered this part as BBa_K395400.
+[[Image:Tokyotech_LacIM1_system.png|left|thumb|300px|Figure3-2-1. ]]
+[[Image:Tokyotech_LacIM1_data.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG.  This work is done by Mitsuhiko Odera ]]
+We confirmed that lacIM1 shows weaker repression than LacIWT.
+(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])