Difference between revisions of "Part:BBa K337033"

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<partinfo>BBa_K337033 short</partinfo>
 
<partinfo>BBa_K337033 short</partinfo>
  
SV40 promoter driving a firefly luciferase reporter gene. Those two elements all rc, CMV promoter near SV40 drives in the opposite direction (fw).  
+
Luc2 reporter gene with CMV promoter cloned behind it. Construction intermediate.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 05:00, 27 October 2010

Luc2_sv40/CMV(fw)

Luc2 reporter gene with CMV promoter cloned behind it. Construction intermediate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1944
    Illegal XhoI site found at 1686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 70
    Illegal NgoMIV site found at 1414
    Illegal NgoMIV site found at 1435
    Illegal NgoMIV site found at 1707
    Illegal AgeI site found at 1138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1320