Difference between revisions of "Part:BBa K395100"

 
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<partinfo>BBa_K395100 short</partinfo>
 
<partinfo>BBa_K395100 short</partinfo>
  
 
This part is GFP reporter which is activated by LuxR and 3OC6HSL, and the promoter is R0062. And we use this part to measure the activity of BBa_R0062.
 
This part is GFP reporter which is activated by LuxR and 3OC6HSL, and the promoter is R0062. And we use this part to measure the activity of BBa_R0062.
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[[IMAGE:Tokyotech LuxR activation promoter assay(R0062).jpg|300px|left|thumb]]
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[[IMAGE:Tokyotech LuxR ractivatio promoter assay2.jpg|300px|left|thumb]]
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In order to measure the activity of R0062, we introduced S03119 (pTetR-rbs-luxR-ter) on pSB3K3 and K395100 into DH5&alpha;.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:22, 27 October 2010

GFP reporter activated by LuxR and 3OC6HSL (R0062:K121013)

This part is GFP reporter which is activated by LuxR and 3OC6HSL, and the promoter is R0062. And we use this part to measure the activity of BBa_R0062.

Tokyotech LuxR activation promoter assay(R0062).jpg
Tokyotech LuxR ractivatio promoter assay2.jpg

In order to measure the activity of R0062, we introduced S03119 (pTetR-rbs-luxR-ter) on pSB3K3 and K395100 into DH5α.











Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 89
    Illegal BamHI site found at 78
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 781