Difference between revisions of "Part:BBa K337023"
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<partinfo>BBa_K337023 short</partinfo>
 
<partinfo>BBa_K337023 short</partinfo>
  
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RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by double Tet operator.
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RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by double Tet operator. Therefor part allows for the regulation of the GOI via the Tet System.
  
 
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<!-- Add more about the biology of this part here

Revision as of 04:21, 27 October 2010

BGH(rc)/shRNA10(rc)/RSV(rc)/CMV(fw)_TetO2(fw)

RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by double Tet operator. Therefor part allows for the regulation of the GOI via the Tet System.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]