|
|
| Line 9: |
Line 9: |
| | | | |
| | <partinfo>BBa_K395008 AddReview number</partinfo> | | <partinfo>BBa_K395008 AddReview number</partinfo> |
| − | <I>iGEM Tokyo_Tech 2010</I>
| |
| | | | |
| − | We designed a new promoter which is repressed by AHL and LuxR complex by changing one base of the existing BioBrick parts (BBa_R0061). <br><br>
| |
| − | In order to characterize K395008, Plux repression promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) and this backbone is pSB6A1. We used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control. <br><br>
| |
| − | Overnight cultures of reporter strains grown at 37 °C containing appropriated antibiotics were diluted at least 1:100 and incubated at 37 °C as fresh cultures. After their OD<sub>590</sub> reached 0.6, added 3OC6HSL. After 3 hours of induction, fluorescence intensity was measured with flow cytometry. <br><br>
| |
| − | After 3 hours of induction by 3OC6HSL, the expression of GFP with 3OC6HSL dropped to 1/3 comparing with the expression without 3OC6HSL. <br><br>
| |
| − |
| |
| − | [[IMAGE:tokyotech_LuxR repression promoter assay(R0061weak).jpg|400px]]
| |
| | | | |
| | <!-- DON'T DELETE --><partinfo>BBa_K395008 EndReviews</partinfo> | | <!-- DON'T DELETE --><partinfo>BBa_K395008 EndReviews</partinfo> |
Latest revision as of 04:04, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K395008
User Reviews
UNIQe0608f4a9e6f6b0b-partinfo-00000000-QINU
No review score entered.
UNIQe0608f4a9e6f6b0b-partinfo-00000002-QINU
