Difference between revisions of "Part:BBa K395008"
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We designed a new promoter which is repressed by AHL and LuxR complex by changing one base of the existing BioBrick parts (BBa_R0061). | We designed a new promoter which is repressed by AHL and LuxR complex by changing one base of the existing BioBrick parts (BBa_R0061). | ||
[[IMAGE:Tokyotech R0061 K395008 graph K395008.jpg|400px|left|thumb]] | [[IMAGE:Tokyotech R0061 K395008 graph K395008.jpg|400px|left|thumb]] | ||
| − | + | In order to characterize this promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) and this backbone is pSB6A1. We used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control. | |
Revision as of 04:02, 27 October 2010
Plux (rep) R0061middle
We designed a new promoter which is repressed by AHL and LuxR complex by changing one base of the existing BioBrick parts (BBa_R0061).
In order to characterize this promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) and this backbone is pSB6A1. We used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]