Difference between revisions of "Part:BBa K415069"
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This is a LuxR/LacO-regulated GFP, LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification, TetR-regulated LuxR production. | This is a LuxR/LacO-regulated GFP, LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification, TetR-regulated LuxR production. | ||
− | This is nearly the same part as [[Part:BBa_K415023]] except that the promoter controlling LuxI expression is a less leaky version of [[Part:BBa_R0065]]. | + | This is nearly the same part as [[Part:BBa_K415023]] except that the promoter controlling LuxI expression is [[Part:BBa_K415031]], a less leaky version of [[Part:BBa_R0065]]. |
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Revision as of 03:40, 27 October 2010
pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fixed-pLux/cI:LuxI
This is a LuxR/LacO-regulated GFP, LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification, TetR-regulated LuxR production. This is nearly the same part as Part:BBa_K415023 except that the promoter controlling LuxI expression is Part:BBa_K415031, a less leaky version of Part:BBa_R0065.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3687
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 747
Illegal BsaI.rc site found at 2954