Difference between revisions of "Part:BBa K395705"

Revision as of 03:21, 27 October 2010

crtW (beta-carotene ketolase) with rbs

CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.

Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.

Fig. 2-1-11 astaxanthin pellet and plate

These parts contain crtW (β-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 632
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 715
Illegal SapI site found at 542
Illegal SapI site found at 554

@@ Line 5: / Line 5: @@
 Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
+[[Image:tokyotech_Fig. 2-1-11 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11 astaxanthin pellet and plate]]
+[[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]]
+[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|450px|Fig. 2-1-1-3.TLC.jpg]]
+These parts contain ''crtW'' (&beta;-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in ''E. coli'' strain MG1655.   (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])