Difference between revisions of "Templates/Cambridge-fireflycolour"

Revision as of 03:18, 27 October 2010

Input:	L-Arabinose
Output:	Light {{{colour}}}

pBad/araC I0500	mutated version of Luciferase/LRE K325210

This part should show similarity to BBa_K325219 [[Part:BBa_K325{{{id}}}:Experience |Add Data]]

Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.

Pictures

Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)

¹Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

@@ Line 12: / Line 12: @@
 promoter (tetR, negative)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 luxR Protein Generator' src='https://parts.igem.org/images/partbypart/icon_reporter.png' height='25' border=0></A></TABLE></html>
-|width="226px" align="center" valign="center"|[[Image:BBa_F2620Icon.png|200px]]
+|width="456px" align="right" valign="center"|<html><img src="https://static.igem.org/mediawiki/parts/c/c3/Cambridge-Eglowli.png" /></html>
 |}
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