Difference between revisions of "Templates/Cambridge-fireflycolour"
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<span style="color:black">This part should show similarity to</span> [https://parts.igem.org/Part:BBa_K325219 BBa_K325219] [[Part:BBa_K325{{{id}}}:Experience |Add Data]] | <span style="color:black">This part should show similarity to</span> [https://parts.igem.org/Part:BBa_K325219 BBa_K325219] [[Part:BBa_K325{{{id}}}:Experience |Add Data]] |
Revision as of 03:10, 27 October 2010
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Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.
Pictures
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3