Difference between revisions of "Templates/Cambridge-fireflycolour"
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'''Description'''<br>
 
'''Description'''<br>
 
This part is based on part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219].  Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part [https://parts.igem.org/Part:BBa_K325209 BBa_K325209] which is the green wild-type luciferase].  Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.   
 
This part is based on part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219].  Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part [https://parts.igem.org/Part:BBa_K325209 BBa_K325209] which is the green wild-type luciferase].  Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.   
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<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
 
<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
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'''Compatibility'''<br>
 
'''Compatibility'''<br>
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br>
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br>
 
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
 
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
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Revision as of 03:08, 27 October 2010

Input: L-Arabinose
Output: Light {{{colour}}}

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210
 BBa F2620Icon.png

This part should show similarity to BBa_K325219     [[Part:BBa_K325{{{id}}}:Experience |Add Data]]       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.


Pictures

Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)

1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3