Difference between revisions of "Templates/Cambridge-fireflycolour"
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<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] | <sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] | ||
<div style="padding: 00px; width: 680px"> | <div style="padding: 00px; width: 680px"> | ||
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| + | ==Sequences== | ||
| + | <partinfo>BBa_K325{{{id}}} SequenceAndFeatures</partinfo> | ||
'''Compatibility'''<br> | '''Compatibility'''<br> | ||
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br> | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br> | ||
Revision as of 03:03, 27 October 2010
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Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.
Pictures
Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]