Difference between revisions of "Part:BBa K325229"

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{{:Templates/Cambridge-fireflycolour|colour=green|substitution=Val239Ile}}
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{{:Templates/Cambridge-fireflycolour|colour=green|substitution=Val239Ile|id=229}}

Revision as of 02:57, 27 October 2010

Input: L-Arabinose
Output: Light (green)

pBad/araC
I0500
mutated version of
Luciferase/LRE
K325210

This part should show similarity to BBa_K325219     Add Data       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence.


Colour of emission

Figure 1 - Comparison of part BBa_K325229 against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3