Difference between revisions of "Part:BBa K349007:Design"

 
(Design Notes)
 
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<partinfo>BBa_K349007 short</partinfo>
 
<partinfo>BBa_K349007 short</partinfo>
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===Design Notes===
 
===Design Notes===
None
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This part has built-in BsaI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.
 
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===Source===
 
===Source===

Latest revision as of 02:51, 27 October 2010

KanR-AB-BsaI (for construction of AB BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 984


Design Notes

This part has built-in BsaI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.

Source

pSB1C3

References