Difference between revisions of "Templates/Cambridge-fireflycolour"
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[[Image:300px-Cambridge-Wed.jpg|thumb|569px|center|'''Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with [https://parts.igem.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [https://parts.igem.org/Part:BBa_K325219 BBa 325219] (red light bulb) ''']] | [[Image:300px-Cambridge-Wed.jpg|thumb|569px|center|'''Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with [https://parts.igem.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [https://parts.igem.org/Part:BBa_K325219 BBa 325219] (red light bulb) ''']] | ||
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<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] | <sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] | ||
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Revision as of 02:50, 27 October 2010
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Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.
Pictures
Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]