Difference between revisions of "Templates/Cambridge-fireflycolour"

Revision as of 02:50, 27 October 2010

Input:	L-Arabinose
Output:	Light {{{colour}}}

pBad/araC I0500	mutated version of Luciferase/LRE K325210

This part should show similarity to BBa_K325219 Add Data

Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.

Pictures

Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)

</center> ¹Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

@@ Line 19: / Line 19: @@
 <div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 660px; align: center">
 <center>
-[[Part:BBa_K325219 |Part Main Page]]
+<span style="color:black">This part should show similarity to</span> [https://parts.igem.org/Part:BBa_K325219 BBa_K325219]&nbsp;&nbsp;&nbsp;&nbsp; [[Part:BBa_K325219:Experience |Add Data]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-[[Part:BBa_K325219:ArabinosetoLight|Arabinose -> Light]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-[[Part:BBa_K325219:LuciferintoLight|Luciferin -> Light]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-[[Part:BBa_K325219:Stability |Effect of pH]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-[[Part:BBa_K325219:Experience |Add Data]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 </center>
 </div><br>
@@ Line 46: / Line 41: @@
 </div>
-'''References'''<br>
-[http://www.ncbi.nlm.nih.gov/pubmed/18949818 '''[1&#x5d;:'''] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,''Life'' '''61''', 6-17.
-</div>
-[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html '''[2&#x5d;:'''] T. Nakatsu ''et al.'' (2006) Structural Basis for the spectral difference in luciferase bioluminescence, ''Nature'' '''440'''(16), 372-376.
-[http://www.ncbi.nlm.nih.gov/pubmed/11457857 '''[3&#x5d;:'''] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, ''The Journal of Biological Chemistry'', '''276'''(39), 36508-36513.