Difference between revisions of "Part:BBa K349006:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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This part has built-in BsaI cutsites surrounding the J00450 RFP cassette. Cutting at the BfuAI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioByte assembly method of Team Alberta 2010.
+
This part has built-in BfuAI cutsites surrounding the J00450 RFP cassette. Cutting at the BfuAI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioByte assembly method of Team Alberta 2010.
  
 
===Source===
 
===Source===

Latest revision as of 02:48, 27 October 2010

RFP-BA-BfuAI (for construction of BA BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has built-in BfuAI cutsites surrounding the J00450 RFP cassette. Cutting at the BfuAI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioByte assembly method of Team Alberta 2010.

Source

pSB1C3

References