Difference between revisions of "Templates/Cambridge-fireflycolour"
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|width="226px" align="center" valign="center"|<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;width:200px'>pBad/araC<br>I0500</TD><TD style='font-size:90%; line-height:100%;text-align:center;'><strong>mutated version of</strong><br>Luciferase/LRE<br>K325210</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
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|width="226px" align="center" valign="center"|<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;width:200px'>pBad/araC<br>I0500</TD><TD style='font-size:90%; line-height:100%;text-align:center;'><strong>mutated version of</strong><br>Luciferase/LRE<br>K325210</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
 
promoter (tetR, negative)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 
promoter (tetR, negative)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 
luxR Protein Generator' src='https://parts.igem.org/images/partbypart/icon_reporter.png' height='25' border=0></A></TABLE></html>
 
luxR Protein Generator' src='https://parts.igem.org/images/partbypart/icon_reporter.png' height='25' border=0></A></TABLE></html>

Revision as of 02:47, 27 October 2010

Input: L-Arabinose
Output: Light {{{colour}}}

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210
 BBa F2620Icon.png

Part Main Page        Arabinose -> Light        Luciferin -> Light        Effect of pH        Add Data       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.


Pictures

Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)

</center> 1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3


References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.

[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.

[http://www.ncbi.nlm.nih.gov/pubmed/11457857 [3]:] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513.