Difference between revisions of "Part:BBa K305011"
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===Usage and Biology===
 
===Usage and Biology===
  
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The SURE system is a stringently controlled expression system that displays both high levels of expression and very little promoter 'leakage'. This makes it ideal for the optimization of heterologous protein expression.
+
The SURE system is a stringently controlled expression system that displays both high levels of expression and very little promoter 'leakage'. This makes it ideal for the optimization of heterologous protein expression. The subtilin producing 'B. subtilis' ATCC 6633 strain senses the presense of the lantibiotic subtilin through the sensor histidine kinase SpaK, which phosphorylates its response regulator SpaR. > autoinduction
  
  

Revision as of 02:46, 27 October 2010

Subtilin inducible expression plasmid for Bacillus subtilis


Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis Bongers, this plasmid was constructed to express proteins present in RCF 10 BioBricks, composed of an RBS followed by a coding sequence, upon subtilin induction.

Usage and Biology

The SURE system is a stringently controlled expression system that displays both high levels of expression and very little promoter 'leakage'. This makes it ideal for the optimization of heterologous protein expression. The subtilin producing 'B. subtilis' ATCC 6633 strain senses the presense of the lantibiotic subtilin through the sensor histidine kinase SpaK, which phosphorylates its response regulator SpaR. > autoinduction


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3141
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3141
    Illegal BglII site found at 3060
    Illegal XhoI site found at 287
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3141
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3141
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3156
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 324



References

<biblio>

  1. Bongers pmid=16332878

</biblio>