Difference between revisions of "Part:BBa K343100:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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This part has an internal pst1 site in the FlhC part of the operon. We also designed a part where the internal pst1 site has been removed by introducing a silent mutation [https://parts.igem.org/Part:BBa_343000 part: BBa_343000] and a composite part [https://parts.igem.org/Part:BBa_K343004 BBa_K343004]
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This part has an internal Pst1 restriction site in the ''flhC'' part of the operon. To remove this we also designed a Pst1-free brick: [https://parts.igem.org/Part:BBa_343000 part: BBa_343000] and a composite part [https://parts.igem.org/Part:BBa_K343004 BBa_K343004], where the restiction site has been removed by introducing a silent mutation.
  
 
===Source===
 
===Source===

Latest revision as of 02:40, 27 October 2010

Flagella master regulating operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 821
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 821
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 821
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 821
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has an internal Pst1 restriction site in the flhC part of the operon. To remove this we also designed a Pst1-free brick: part: BBa_343000 and a composite part BBa_K343004, where the restiction site has been removed by introducing a silent mutation.

Source

It is a natural part of the E.coli genome

References