Difference between revisions of "Part:BBa K337051"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K337051 short</partinfo>
 
<partinfo>BBa_K337051 short</partinfo>
  
−
designed, synthetic shRNA-like miRNA followed by BGH terminator, both rc
+
designed, synthetic microRNA (reverse complementary, hsa-mir122 adopted sequence) put behind BGH terminator (reverse complementary) (BB-2, RFC12).
 +
Used as template in the synthesis of any synthetic microRNA of the users' choice.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:28, 27 October 2010

BGH(rc)/shRNA10(rc)

designed, synthetic microRNA (reverse complementary, hsa-mir122 adopted sequence) put behind BGH terminator (reverse complementary) (BB-2, RFC12). Used as template in the synthesis of any synthetic microRNA of the users' choice.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]